Sains Malaysiana 53(11)(2024): 3651-3661

http://doi.org/10.17576/jsm-2024-5311-09

Sintesis dan Pencirian Zarah Silika Berongga dalam Aplikasi Biosensor DNA

(Synthesis and Characterization of Hollow Silica Spheres as DNA Biosensor Application)

EDA YUHANA ARIFFIN¹, LEE YOOK HENG² & SITI AISHAH HASBULLAH^2,*

¹Chemistry Section, School of Distance Education, Universiti Sains Malaysia, 11800 USM Penang, Malaysia

²Department of Chemical Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

Diserahkan: 21 Mei 2024/Diterima: 13 Ogos 2024

Abstrak

Pembangunan model biosensor DNA berasaskan zarah silika berongga sebagai tapak pemegunan DNA boleh digunakan di dalam aplikasi biosensor DNA. Zarah silika berongga berjaya disintesis dan pencirian dijalankan dengan menggunakanmikroskop elektron pengimbasan pelepasan medan (FESEM), kalorimeter imbasan pembeza (DSC), penganalisis saiz zarah dan pembelauan sinar-X (XRD). Isi padu rongga, luas permukaan khusus dan penyebaran saiz rongga diukur dengan menggunakan Micrometric ASAP 2020 melalui kaedah Brunauer-Emmett-Teller(BET)/Barret-Joyner-Halenda(BJH). Surfaktan Tween 20, Triton-X dan CTAB dengan suhu dan masa tindak balas yang berbeza digunakan dalam penghasilan zarah silika berongga. Berdasarkan analisis FESEM dan BET/BJH, zarah silika berongga yang disintesis dengan menggunakan surfaktan Tween 20 pada suhu 27 °C dan tindak balas dijalankan selama 8 hari mempunyai luas permukaan yang paling besar. Zarah silika berongga ini digunakan dalam pemegunan prob DNA E. coli dan didapati biosensor ini menunjukkan kebolehpilihan yang tinggi terhadap DNA sasaran E. coli. Ini disebabkan oleh zarah silika berongga mempunyai tapak pemegunan prob DNA yang tinggi dan kadar penghibridan juga meningkat. Kajian ini sangat penting dalam menghasilkan zarah silika berongga sebagai tapak pemegun DNA bagi biosensor DNA yang dibangunkan.

Kata kunci: Biosensor DNA; pencirian; sintesis; zarah silika berongga

Abstract

Development of DNA biosensor model based on hollow silica spheres as DNA immobilization sites can be used for DNA biosensor application. Hollow silica spheres were successfully synthesized and characterized using field emission scanning electron microscopy (FESEM), differential scanning calorimetry (DSC), particle size analyzer and X-ray diffraction (XRD). Hollow volume, specific surface area and hollow size distribution was measured using Micrometer ASAP 2020 through Brunauer-Emmett-Teller(BET)/Barret-Joyner-Halenda(BJH) method. Tween 20 surfactant, Triton-X and CTAB with different temperature and different reaction time was used in synthesizing hollow silica spheres. Based on FESEM and BET/BJH analysis, hollow silica spheres synthesized using Tween 20 surfactant at temperature 27 °C and the reaction carried out for 8 days have the largest surface area. Hollow silica spheres was used in E. coli DNA probe immobilization and it was found that this biosensor showed high selectivity against E. coli DNA target. This is due to the hollow silica particles having high DNA probe immobilization sites and increased hybridization rates. This new finding is so valuable in making hollow silica spheres as DNA immobilisation sites for the developed DNA biosensor.

Keywords: Characterisation; DNA biosensor; hollow silica spheres; synthesis

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*Pengarang untuk surat-menyurat; email: aishah80@ukm.edu.my